White Button Mushroom (Agaricus bisporus) Liquid Culture for Mushroom Cultivation on Grain or Agar

Colonized like a beast in rye berries. Starting to fruit now in an all-in-one wood lover bag.

Update 3/10/26 I messaged the seller regarding this issue and received a new batch right away. This batch did in fact grow really well. The seller was quick to try to resolve the issue when asked so changing the 1 star to 5 stars. I purchased the 10 ml syringe of Red Reishi liquid culture in August 2025. Along with 3 other LC syringes from other distributors. Split all of them between grain and more LC. All of the others have grown and produced. But not the Reishi. It appears to be dead. No growth in the grain. No growth in the liquid culture. It is now mid January. I’ve tried several times from the liquid culture on grain and agar. Nothing.

Received this, lions mane and king oysters. I attempted agar inoculation when I first received. after 15 days no growth was observed. I attempted a second round, ever changing my agar recipe from malts yeast agar to potato dextrose. Still nothing, so decided to throw it under magnification. These images are the result of what I found. It looks like there was possible growth in the syringe, however, it seems contamination has taken place. Plates were inoculated in a positive pressure clean room with laminar flow hood and strict aseptic technique followed. I’d be really surprised if the contam was a result of my inoculation environment. Possibly the syringe froze during transport, seeing as how it is winter… still a big disappointment. I’d say be careful and aware of when you’re ordering these to make sure that you are not paying for what will be received as a ruined spore syringe. Obviously there is a plethora of organic material… just nothing active apparently🫠😢

Was able to produce great oysters!! Expanded fine and everything is good!

I was not impressed with the amount of mycelium present as the syringe did not contain much at all compared to other reviewers. Never the less I planted it; some in used coffee grounds with ground wheat flakes and the other half in hardwood pellets and ground wheat flakes. Both appear to be growing a healthy amount of mycelium. Since there was not much mycelium in the syringe compared to the growth medium I was unable to instill any into a log as I had hoped.

The most dependable, trouble free oyster strain. Clean in grain and LC.

I ordered two spore syringes: one Portobello and one White Button. Unfortunately, the Portobello syringe was contaminated—the mycelium inside had a clumpy, slimy texture, clearly unsuitable for use. I’ve contacted the seller and am waiting for a refund for this syringe. On the positive side, the White Button syringe was perfect, with clean and healthy mycelium. Update: The refund process worked perfectly, and the seller took the time to address my concerns in a very professional and friendly manner. I am happy to update my review to 5 stars.

I bought 3 strains, and made a large batch of agar solution to work with, worked with a sill air box and a flow hood. The white enokitake took wonderful hold, rizomorphic mycelium. 10 dishes, no contam. Wine caps, same thing maybe a little less aggressive growth. With the lions mane, every single dish had bacterial contamination which leads me to believe the stringe was the source of the issue. Unfortunately it was the lions mane i was most excited for 😅 If it weren’t for that I’d recommend this seller but this was the first case ive ever had with a contaminated syringe.